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Intramolecular binding mode of the C-terminus of Escherichia coli single-stranded DNA binding protein determined by nuclear magnetic resonance spectroscopy

Shishmarev, Dmitry; Wang, Yao; Mason, Claire E; Su, Xun-Cheng; Oakley, Aaron; Graham, Bim; Dixon, Nicholas Edward; Otting, Gottfried; Huber, Thomas

Description

Single-stranded DNA (ssDNA) binding protein (SSB) is an essential protein to protect ssDNA and recruit specific ssDNA-processing proteins. Escherichia coli SSB forms a tetramer at neutral pH, comprising a structurally well-defined ssDNA binding domain (OB-domain) and a disordered C-terminal domain (C-domain) of ∼64 amino acid residues. The C-terminal eight-residue segment of SSB (C-peptide) has been shown to interact with the OB-domain, but crystal structures failed to reveal any electron...[Show more]

CollectionsANU Research Publications
Date published: 2014
Type: Journal article
URI: http://hdl.handle.net/1885/62900
Source: Nucleic Acids Research
DOI: 10.1093/nar/gkt1238

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