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DNA-based vaccines protect against zoonotic schistosomiasis in water buffalo

Da'Dara, Akram A.; Li, Yue-Sheng; Xiong, Tie; Zhou, Jie; Williams, Gail; McManus, Donald P.; Feng, Zeng; Yu, Xin-Ling; Gray, Darren; Harn, Donald A.

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Schistosomiasis japonica is an endemic, zoonotic disease of major public health importance in China where water buffaloes account for approximately 75% of disease transmission. Interventions that reduce schistosome infection in water buffaloes will enhance their health simultaneously reducing disease transmission to humans. While chemotherapy has proved successful, it requires continued time consuming and expensive mass treatments. A more sustainable option would be development of vaccines that...[Show more]

dc.contributor.authorDa'Dara, Akram A.
dc.contributor.authorLi, Yue-Sheng
dc.contributor.authorXiong, Tie
dc.contributor.authorZhou, Jie
dc.contributor.authorWilliams, Gail
dc.contributor.authorMcManus, Donald P.
dc.contributor.authorFeng, Zeng
dc.contributor.authorYu, Xin-Ling
dc.contributor.authorGray, Darren
dc.contributor.authorHarn, Donald A.
dc.date.accessioned2015-12-10T23:06:49Z
dc.identifier.issn0264-410X
dc.identifier.urihttp://hdl.handle.net/1885/62818
dc.description.abstractSchistosomiasis japonica is an endemic, zoonotic disease of major public health importance in China where water buffaloes account for approximately 75% of disease transmission. Interventions that reduce schistosome infection in water buffaloes will enhance their health simultaneously reducing disease transmission to humans. While chemotherapy has proved successful, it requires continued time consuming and expensive mass treatments. A more sustainable option would be development of vaccines that reduce transmission of S. japonicum from bovines to replace bovine chemotherapy. We performed two randomized double blind trials in water buffaloes to determine if DNA vaccines encoding triose-phosphate isomerase (SjCTPI), or the tetraspanin 23 kDa integral membrane protein (SjC23), alone or fused to bovine heat shock protein 70 (Hsp70) could induce a level of immunity conducive to long-term sustainable control. Groups of water buffaloes (15/group) received three intramuscular injections, 4 weeks apart. Booster immunizations were co-administered with a plasmid DNA encoding IL-12. Four weeks after the last injection, water buffaloes were challenged with 1000 cercariae, and vaccine efficacy analyzed 8 weeks later. Water buffaloes vaccinated with SjCTPI-Hsp70 or SjCTPI plasmids had worm burdens reduced by 51.2% and 41.5%, respectively. Importantly, fecal miracidial hatching was reduced by 52.1% and 33.2% respectively compared to control vaccinated water buffaloes. Vaccination with SjC23-Hsp70 and SjC23 plasmids reduced worm burdens by 50.9% and 45.5%, respectively, and fecal miracidial hatching by 52.0% and 47.4%. A mathematical model of schistosome transmission predicts that schistosome vaccines capable of reducing water buffaloes' fecal egg output by 45%, alone or in conjunction with praziquantel treatment, will lead to a significant reduction in transmission of schistosomiasis. Both DNA vaccines tested here exceed this hypothetical level. Indeed, mathematical modeling of SjCTPI-Hsp70 and SjC23-Hsp70 alone and in conjunction with human chemotherapy showed a significant reduction in transmission almost to the point of elimination.
dc.publisherElsevier
dc.sourceVaccine
dc.subjectKeywords: DNA vaccine; heat shock protein 70; ivermectin; membrane protein; praziquantel; tetraspanin; triosephosphate isomerase; animal experiment; animal model; article; buffalo; controlled study; cow; disease transmission; drug efficacy; female; male; nonhuman; DNA vaccine; IL-12; Schistosoma japonicum; SjC23-Hsp70; SjCTPI-Hsp70; Water buffaloes
dc.titleDNA-based vaccines protect against zoonotic schistosomiasis in water buffalo
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume26
dc.date.issued2008
local.identifier.absfor111706 - Epidemiology
local.identifier.ariespublicationU3488905xPUB740
local.type.statusPublished Version
local.contributor.affiliationDa'Dara , Akram A , Harvard School of Public Health
local.contributor.affiliationLi, Yue-Sheng, Queensland Institute of Medical Research
local.contributor.affiliationXiong, Tie, Hunan Institute of Parasitic Diseases
local.contributor.affiliationZhou, Jie, Hunan Institute of Parasitic Diseases
local.contributor.affiliationWilliams, Gail, University of Queensland
local.contributor.affiliationMcManus, Donald P, Queensland Institute of Medical Research
local.contributor.affiliationFeng, Zeng, Chinese Centre for Disease Control and Prevention
local.contributor.affiliationYu, Xin-Ling, Hunan Institute of Parasitic Diseases
local.contributor.affiliationGray, Darren, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHarn , Donald A , Harvard School of Public Health
local.description.embargo2037-12-31
local.bibliographicCitation.issue29-30
local.bibliographicCitation.startpage3617
local.bibliographicCitation.lastpage3625
local.identifier.doi10.1016/j.vaccine.2008.04.080
dc.date.updated2015-12-10T08:56:49Z
local.identifier.scopusID2-s2.0-45049088311
CollectionsANU Research Publications

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