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Substrate-induced Assembly of Methanococcoides burtonii D-Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Dimers into Decamers

Alonso, Hernan; Blayney, Michelle J; Beck, Jennifer; Whitney, Spencer

Description

Like many enzymes, the biogenesis of the multi-subunit CO2-fixing enzyme ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) in different organisms requires molecular chaperones. When expressed in Escherichia coli, the large (L) subunits of the Rubisco from the archaeabacterium Methanococcoides burtonii assemble into functional dimers (L2). However, further assembly into pentamers of L2 (L10) occurs when expressed in tobacco chloroplasts or E. coli producing RuBP. In vitro analyses...[Show more]

CollectionsANU Research Publications
Date published: 2009
Type: Journal article
URI: http://hdl.handle.net/1885/62562
Source: Journal of Biological Chemistry
DOI: 10.1074/jbc.M109.050989

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