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Three-dimensional protein fold determination from backbone amide pseudocontact shifts generated by lanthanide tags at multiple sites

Yagi, Hiromasa; Pilla, Kala; Maleckis, Ansis; Graham, Bim; Otting, Gottfried; Huber, Thomas

Description

Site-specific attachment of paramagnetic lanthanide ions to a protein generates pseudocontact shifts (PCS) in the nuclear magnetic resonance (NMR) spectra of the protein that are easily measured as changes in chemical shifts. By labeling the protein with

dc.contributor.authorYagi, Hiromasa
dc.contributor.authorPilla, Kala
dc.contributor.authorMaleckis, Ansis
dc.contributor.authorGraham, Bim
dc.contributor.authorOtting, Gottfried
dc.contributor.authorHuber, Thomas
dc.date.accessioned2015-12-10T23:03:12Z
dc.identifier.issn0969-2126
dc.identifier.urihttp://hdl.handle.net/1885/62069
dc.description.abstractSite-specific attachment of paramagnetic lanthanide ions to a protein generates pseudocontact shifts (PCS) in the nuclear magnetic resonance (NMR) spectra of the protein that are easily measured as changes in chemical shifts. By labeling the protein with
dc.publisherCell Press
dc.sourceStructure
dc.subjectKeywords: amide; chaperone; chaperone ERp29; lanthanide; unclassified drug; amino acid sequence; article; carboxy terminal sequence; crystal structure; nonhuman; priority journal; protein domain; protein folding; protein structure; proton nuclear magnetic resonance
dc.titleThree-dimensional protein fold determination from backbone amide pseudocontact shifts generated by lanthanide tags at multiple sites
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume21
dc.date.issued2013
local.identifier.absfor030403 - Characterisation of Biological Macromolecules
local.identifier.ariespublicationu4005981xPUB665
local.type.statusPublished Version
local.contributor.affiliationYagi, Hiromasa, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationPilla, Kala, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationMaleckis, Ansis, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationGraham, Bim, Monash Institute of Phamaceutical Sciences
local.contributor.affiliationHuber, Thomas, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationOtting, Gottfried, College of Physical and Mathematical Sciences, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue6
local.bibliographicCitation.startpage883
local.bibliographicCitation.lastpage890
local.identifier.doi10.1016/j.str.2013.04.001
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2016-02-24T10:25:38Z
local.identifier.scopusID2-s2.0-84878871491
local.identifier.thomsonID000320739800005
CollectionsANU Research Publications

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