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Three-dimensional protein fold determination from backbone amide pseudocontact shifts generated by lanthanide tags at multiple sites

Yagi, Hiromasa; Pilla, Kala; Maleckis, Ansis; Graham, Bim; Otting, Gottfried; Huber, Thomas

Description

Site-specific attachment of paramagnetic lanthanide ions to a protein generates pseudocontact shifts (PCS) in the nuclear magnetic resonance (NMR) spectra of the protein that are easily measured as changes in chemical shifts. By labeling the protein with

CollectionsANU Research Publications
Date published: 2013
Type: Journal article
URI: http://hdl.handle.net/1885/62069
Source: Structure
DOI: 10.1016/j.str.2013.04.001

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