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Frost tolerance and ice formation in Pinus radiata needles: ice management by the endodermis and transfusion tissues

Roden, James; Canny, Martin; Huang, Cheng; Ball, Marilyn


Conifers are among the most frost tolerant tree species. Cryo-scanning electron microscopy (cryo-SEM) was used to visualise ice formation in pine needles to better understand how conifer leaves manage extracellular ice. Acclimated and unacclimated needles of Pinus radiata (D.Don) were subjected to freezing treatments (at a rate of 2°Ch-1), tested for electrolyte leakage and sampled for cryo-SEM analysis. Half maximal electrolyte leakage occurred at -4 and -12°C for unacclimated and acclimated...[Show more]

CollectionsANU Research Publications
Date published: 2009
Type: Journal article
Source: Functional Plant Biology
DOI: 10.1071/FP08247


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