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W-band orientation selective DEER measurements on a Gd 3+ /nitroxide mixed-labeled protein dimer with a dual mode cavity

Kaminker, Ilia; Tkach, Igor; Manukovsky, Nurit; Yagi, Hiromasa; Bennati, Marina; Goldfarb, Daniella; Otting, Gottfried; Huber, Thomas


Double electron-electron resonance (DEER) at W-band (95 GHz) was applied to measure the distance between a pair of nitroxide and Gd3+ chelate spin labels, about 6 nm apart, in a homodimer of the protein ERp29. While high-field DEER measurements on systems with such mixed labels can be highly attractive in terms of sensitivity and the potential to access long distances, a major difficulty arises from the large frequency spacing (about 700 MHz) between the narrow, intense signal of the Gd3+...[Show more]

CollectionsANU Research Publications
Date published: 2013
Type: Journal article
Source: Journal of Magnetic Resonance
DOI: 10.1016/j.jmr.2012.11.028


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