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Sequence-Specific and Stereospecific Assignment of Methyl Groups Using Paramagnetic Lanthanides

John, Michael; Schmitz, Christophe; Park, Ah Young; Dixon, Nicholas; Otting, Gottfried; Huber, Thomas

Description

Pseudocontact shifts (PCSs) induced by a site-specifically bound paramagnetic lanthanide ion are shown to provide fast access to sequence-specific resonance assignments of methyl groups in proteins of known three-dimensional structure. Stereospecific assignments of Val and Leu methyls are obtained as well as resonance assignments of all other methyls, including Met εCH3 groups. No prior assignments of the diamagnetic protein are required nor are experiments that transfer magnetization between...[Show more]

dc.contributor.authorJohn, Michael
dc.contributor.authorSchmitz, Christophe
dc.contributor.authorPark, Ah Young
dc.contributor.authorDixon, Nicholas
dc.contributor.authorOtting, Gottfried
dc.contributor.authorHuber, Thomas
dc.date.accessioned2015-12-10T23:01:37Z
dc.identifier.issn0002-7863
dc.identifier.urihttp://hdl.handle.net/1885/61679
dc.description.abstractPseudocontact shifts (PCSs) induced by a site-specifically bound paramagnetic lanthanide ion are shown to provide fast access to sequence-specific resonance assignments of methyl groups in proteins of known three-dimensional structure. Stereospecific assignments of Val and Leu methyls are obtained as well as resonance assignments of all other methyls, including Met εCH3 groups. No prior assignments of the diamagnetic protein are required nor are experiments that transfer magnetization between the methyl groups and the protein backbone. Methyl Cz-exchange experiments were designed to provide convenient access to PCS measurements in situations where a paramagnetic lanthanide is in exchange with a diamagnetic lanthanide. In the absence of exchange, simultaneous 13C-HSQC assignments and PCS measurements are delivered by the newly developed program Possum. The approaches are demonstrated with the complex between the N-terminal domain of the subunit ε and the subunit θ of the Escherichia coli DNA polymerase III.
dc.publisherAmerican Chemical Society
dc.sourceJournal of the American Chemical Society
dc.subjectKeywords: Molecular structure; Proteins; Rare earth elements; Resonance; Paramagnetic lanthanide; Stereospecific assignments; Stereochemistry; DNA directed DNA polymerase gamma; lanthanide; methyl group; amino terminal sequence; article; electron spin resonance; en
dc.titleSequence-Specific and Stereospecific Assignment of Methyl Groups Using Paramagnetic Lanthanides
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume129
dc.date.issued2007
local.identifier.absfor060102 - Bioinformatics
local.identifier.ariespublicationU4217927xPUB630
local.type.statusPublished Version
local.contributor.affiliationJohn, Michael, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationSchmitz, Christophe, University of Queensland
local.contributor.affiliationPark, Ah Young, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationDixon, Nicholas, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationHuber, Thomas, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationOtting, Gottfried, College of Physical and Mathematical Sciences, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue44
local.bibliographicCitation.startpage13749
local.bibliographicCitation.lastpage13757
local.identifier.doi10.1021/ja0744753
dc.date.updated2015-12-10T08:28:49Z
local.identifier.scopusID2-s2.0-35949002912
CollectionsANU Research Publications

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