King, Gordon J.; Jones, Alun; Kobe, Bostjan; Huber, Thomas; Mouradov, Dmitri; Hume, D A; Ross, Ian R.
Cross-linking can be used to identify spatial relationships between amino acids in proteins or protein complexes. A rapid and sensitive method for identifying the site of protein cross-linking using dithiobis(sulfosuccinimidyl propionate) (DTSSP) is presented and illustrated with experiments using murine cortactin, actin and acyl-CoA thioesterase. A characteristic 66 Da doublet, which arises from the asymmetric fragmentation of the disulfide of DTSSP-modifled peptides, is observed in the mass...[Show more]
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.