Evolutionary optimization of computationally designed enzymes: Kemp eliminases of the KE07 series
Understanding enzyme catalysis through the analysis of natural enzymes is a daunting challenge-their active sites are complex and combine numerous interactions and catalytic forces that are finely coordinated. Study of more rudimentary (wo)man-made enzymes provides a unique opportunity for better understanding of enzymatic catalysis. KE07, a computationally designed Kemp eliminase that employs a glutamate side chain as the catalytic base for the critical proton abstraction step and an apolar...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of Molecular Biology|
|01_Khersonsky_Evolutionary_optimization_of_2010.pdf||5.73 MB||Adobe PDF||Request a copy|
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.