Evolutionary optimization of computationally designed enzymes: Kemp eliminases of the KE07 series
Khersonsky, Olga; Rothlisberger, Daniela; Dym, Orly; Albeck, Shira; Jackson, Colin; Baker, David; Tawfik, Dan S
Description
Understanding enzyme catalysis through the analysis of natural enzymes is a daunting challenge-their active sites are complex and combine numerous interactions and catalytic forces that are finely coordinated. Study of more rudimentary (wo)man-made enzymes provides a unique opportunity for better understanding of enzymatic catalysis. KE07, a computationally designed Kemp eliminase that employs a glutamate side chain as the catalytic base for the critical proton abstraction step and an apolar...[Show more]
Collections | ANU Research Publications |
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Date published: | 2010 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/61228 |
Source: | Journal of Molecular Biology |
DOI: | 10.1016/j.jmb.2009.12.031 |
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01_Khersonsky_Evolutionary_optimization_of_2010.pdf | 5.73 MB | Adobe PDF | Request a copy |
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