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Evolutionary optimization of computationally designed enzymes: Kemp eliminases of the KE07 series

Khersonsky, Olga; Rothlisberger, Daniela; Dym, Orly; Albeck, Shira; Jackson, Colin; Baker, David; Tawfik, Dan S


Understanding enzyme catalysis through the analysis of natural enzymes is a daunting challenge-their active sites are complex and combine numerous interactions and catalytic forces that are finely coordinated. Study of more rudimentary (wo)man-made enzymes provides a unique opportunity for better understanding of enzymatic catalysis. KE07, a computationally designed Kemp eliminase that employs a glutamate side chain as the catalytic base for the critical proton abstraction step and an apolar...[Show more]

CollectionsANU Research Publications
Date published: 2010
Type: Journal article
Source: Journal of Molecular Biology
DOI: 10.1016/j.jmb.2009.12.031


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