Skip navigation
Skip navigation

Testing the evolvability of an insect carboxylesterase for the detoxification of synthetic pyrethroid insecticides

Coppin, Christopher W; Jackson, Colin; Sutherland, Tara; Hart, Peter J; Devonshire, Alan L; Russell, Robyn J; Oakeshott , John


Esterases have been implicated in metabolic resistance to synthetic pyrethroids in several insect species but little is yet known of the molecular basis for these effects. In this work modern directed evolution technology was used to test to what extent it is possible to genetically enhance the pyrethroid hydrolytic activity of the E3 carboxylesterase from the blowfly Lucilia cuprina. High throughput screening of a random mutant library with individual stereoisomers of fluorogenic analogues of...[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
Source: Insect Biochemistry and Molecular Biology
DOI: 10.1016/j.ibmb.2012.01.004


File Description SizeFormat Image
01_Coppin_Testing_the_evolvability_of_an_2012.pdf1.07 MBAdobe PDF    Request a copy

Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator