Testing the evolvability of an insect carboxylesterase for the detoxification of synthetic pyrethroid insecticides
Esterases have been implicated in metabolic resistance to synthetic pyrethroids in several insect species but little is yet known of the molecular basis for these effects. In this work modern directed evolution technology was used to test to what extent it is possible to genetically enhance the pyrethroid hydrolytic activity of the E3 carboxylesterase from the blowfly Lucilia cuprina. High throughput screening of a random mutant library with individual stereoisomers of fluorogenic analogues of...[Show more]
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|Source:||Insect Biochemistry and Molecular Biology|
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