High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites
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Ozawa, Kiyoshi; Loscha, Karin; Kuppan, Kekini V; Loh, Choy; Dixon, Nicholas Edward; Otting, Gottfried
Description
Using aminoacyl-tRNA synthetase/suppressor tRNA pairs derived from Methanocaldococcus jannaschii, an Escherichia coli cell-free protein production system affords proteins with site-specifically incorporated unnatural amino acids (UAAs) in high yields through the use of optimized amber suppressor tRNACUAopt and optimization of reagent concentrations. The efficiency of the cell-free system allows the incorporation of trifluoromethyl-phenylalanine using a polyspecific synthetase evolved previously...[Show more]
Collections | ANU Research Publications |
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Date published: | 2012 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/60845 |
Source: | Biochemical and Biophysical Research Communications |
DOI: | 10.1016/j.bbrc.2012.01.069 |
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