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Multiple-site labeling of proteins with unnatural amino acids

Loscha, Karin; Herlt, Anthony; Qi, Ruhu; Ozawa, Kiyoshi; Otting, Gottfried; Huber, Thomas


A cell-free protein synthesis system from which the release factor RF1 has been selectively removed enables the facile incorporation of unnatural amino acids into proteins at difficult and multiple sites by optimized use of orthogonal tRNA/aminoacyl-tRNA

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
Source: Angewandte Chemie International Edition
DOI: 10.1002/anie.201108275


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