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Nanometer-range distance measurement in a protein using Mn 2+ tags

Banerjee, Debamalya; Yagi, Hiromasa; Goldfarb, Daniella; Otting, Gottfried; Huber, Thomas


Pulse electron paramagnetic resonance measurements of long-range (nm scale) distances between spin labels site-specifically attached to biomacromolecules have proven highly effective in structural studies. The most commonly used spin labels are stable nitroxide radicals, and measurements are usually carried out at X-band frequencies (∼9.5 GHz, 0.35 T). Higher magnetic fields open new possibilities for distance measurements with increased sensitivity using alternative spin labels containing...[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
Source: Journal of Physical Chemistry Letters
DOI: 10.1021/jz201521d


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