Fermentative glycolysis with purified Escherichia coli enzymes for in vitro ATP production and evaluating an engineered enzyme
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Stevenson, Bradley; Liu, Jian-Wei; Kuchel, Philip W; Ollis, David
Description
Each of the twelve enzymes for glycolytic fermentation, eleven from Escherichia coli and one from Saccharomyces cerevisiae, have been over-expressed in E. coli and purified with His-tags. Simple assays have been developed for each enzyme and they have been assembled for fermentation of glucose to ethanol. Phosphorus-31 NMR revealed that this in vitro reaction accumulates fructose 1,6-bisphosphate while recycling the cofactors NAD+ and ATP. This reaction represents a defined ATP-regeneration...[Show more]
Collections | ANU Research Publications |
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Date published: | 2012 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/60743 |
Source: | Journal of Biotechnology |
DOI: | 10.1016/j.jbiotec.2011.09.019 |
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01_Stevenson_Fermentative_glycolysis_with_2012.pdf | 767.1 kB | Adobe PDF | Request a copy |
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