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A rapid, whole-tissue determination of the functional fraction of PSII after photoinhibition of leaves based on flash-induced P700 redox kinetics

Losciale, Pasquale; Oguchi, Riichi; Hendrickson, Luke; Hope, Alexander B; Corelli-Grappadelli, Luca; Chow, Wah S (Fred)

Description

Assaying the number of functional PSII complexes by the oxygen yield from leaf tissue per saturating, single-turnover flash, assuming that each functional PSII evolves one oxygen molecule after four flashes, is one of the most direct methods but time-consuming. The ratio of variable to maximum Chl fluorescence yield (Fv/Fm) in leaves can be correlated with the oxygen yield per flash during a progressive loss of PSII activity associated with high-light stress and is rapid and non-intrusive, but...[Show more]

CollectionsANU Research Publications
Date published: 2008
Type: Journal article
URI: http://hdl.handle.net/1885/60575
Source: Physiologia Plantarum
DOI: 10.1111/j.1399-3054.2007.01000.x

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