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Antimicrobial peptide immunity protects human nasal and auricular cartilage against infection

Warnke, Patrick H.; Russo, Paul; Hopfenziz, Martin; Kurz, Bodo; Becker, Stephan T; Sherry, Eugene; Springer, Ingo; Sivananthan, Sureshan

Description

Background: Despite being impervious to surveillance by the adaptive immune system because of its lack of vascularity, infection of the nasal and auricular cartilage after surgery such as rhinoplasty or otoplasty is rare. Why is this so? Our goal was to determine whether the expression of antimicrobial peptides provides a previously unrecognized nonepithelial layer of innate immune defense within the nasal and auricular cartilage. Materials and Methods: Seven samples of nasal septum cartilage...[Show more]

dc.contributor.authorWarnke, Patrick H.
dc.contributor.authorRusso, Paul
dc.contributor.authorHopfenziz, Martin
dc.contributor.authorKurz, Bodo
dc.contributor.authorBecker, Stephan T
dc.contributor.authorSherry, Eugene
dc.contributor.authorSpringer, Ingo
dc.contributor.authorSivananthan, Sureshan
dc.date.accessioned2015-12-10T22:57:09Z
dc.identifier.issn1049-2275
dc.identifier.urihttp://hdl.handle.net/1885/60532
dc.description.abstractBackground: Despite being impervious to surveillance by the adaptive immune system because of its lack of vascularity, infection of the nasal and auricular cartilage after surgery such as rhinoplasty or otoplasty is rare. Why is this so? Our goal was to determine whether the expression of antimicrobial peptides provides a previously unrecognized nonepithelial layer of innate immune defense within the nasal and auricular cartilage. Materials and Methods: Seven samples of nasal septum cartilage and 2 biopsies from auricular cartilage grafts were harvested during rhinoplasty and otoplasty procedures. Ten cadaveric samples of auricular and 9 samples of nasal cartilage were also obtained. Immunohistochemical staining was directed against the human β-defensin antimicrobial peptides (hBD) 1, 2, and 3. A semiquantitative analysis was performed to measure immunoreactivity. Results: All 3 human β-defensins were detected along the perichondral line and within the cartilage matrix in the nasal and auricular samples. Areas with positive immunohistochemical staining were also detected within chondrocyte cytoplasm. Conclusions: We provide the first evidence of antimicrobial peptide expression (hBD-1, -2 and -3) within the perichondrium and cartilage matrix layers of the nasal and auricular cartilage. This previously unrecognized innate immune function of perichondrocytes and chondrocytes may explain the resistance of the nasal and auricular cartilage to infection after surgical procedures despite the absence of a vascular system.
dc.publisherLippincott Williams & Wilkins
dc.sourceJournal of Craniofacial Surgery
dc.subjectKeywords: beta defensin; article; cadaver; cartilage cell; ear cartilage; ear surgery; enzyme immunoassay; human; immunology; microbiology; nose reconstruction; nose septum; surgical infection; beta-Defensins; Cadaver; Chondrocytes; Ear Cartilage; Humans; Immunoenz Antimicrobial peptides; Auriculoplasty; Cartilage; Defensin; Human ß-defensin; Innate immunity; Rhinoplasty
dc.titleAntimicrobial peptide immunity protects human nasal and auricular cartilage against infection
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume21
dc.date.issued2010
local.identifier.absfor110323 - Surgery
local.identifier.ariespublicationf2965xPUB546
local.type.statusPublished Version
local.contributor.affiliationWarnke, Patrick H., Bond University
local.contributor.affiliationRusso, Paul, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHopfenziz, Martin, Univeristy of Kiel
local.contributor.affiliationKurz, Bodo, University of Kiel
local.contributor.affiliationBecker, Stephan T, Christian-Albrechts-University Kiel
local.contributor.affiliationSherry, Eugene, Sydney Private Hospital
local.contributor.affiliationSpringer, Ingo, Bond University
local.contributor.affiliationSivananthan, Sureshan, University College London
local.description.embargo2037-12-31
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage198
local.bibliographicCitation.lastpage201
local.identifier.doi10.1097/SCS.0b013e3181c50fc2
dc.date.updated2016-02-24T08:30:22Z
local.identifier.scopusID2-s2.0-77649212944
CollectionsANU Research Publications

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