Skip navigation
Skip navigation

Extension, single-locus conversion and physical mapping of sex chromosome sequences identify the Z microchromosome and pseudo-autosomal region in a dragon lizard, Pogona vitticeps

Quinn, Alexander E; Ezaz, Tariq; Sarre, Stephen; Graves, Jennifer; Georges, Arthur

Description

Distribution of temperature-dependent sex determination (TSD) and genotypic sex determination (GSD) across the phylogeny of dragon lizards implies multiple independent origins of at least one, and probably both, modes of sex determination. Female Pogona vitticeps are the heterogametic sex, but ZZ individuals reverse to a female phenotype at high incubation temperatures. We used reiterated genome walking to extend Z and W chromosome-linked amplified fragment length polymorphism (AFLP) markers,...[Show more]

dc.contributor.authorQuinn, Alexander E
dc.contributor.authorEzaz, Tariq
dc.contributor.authorSarre, Stephen
dc.contributor.authorGraves, Jennifer
dc.contributor.authorGeorges, Arthur
dc.date.accessioned2015-12-10T22:57:09Z
dc.identifier.issn0018-067X
dc.identifier.urihttp://hdl.handle.net/1885/60530
dc.description.abstractDistribution of temperature-dependent sex determination (TSD) and genotypic sex determination (GSD) across the phylogeny of dragon lizards implies multiple independent origins of at least one, and probably both, modes of sex determination. Female Pogona vitticeps are the heterogametic sex, but ZZ individuals reverse to a female phenotype at high incubation temperatures. We used reiterated genome walking to extend Z and W chromosome-linked amplified fragment length polymorphism (AFLP) markers, and fluorescence in situ hybridization for physical mapping. One extended fragment hybridized to both W and Z microchromosomes, identifying the Z microchromosome for the first time, and a second hybridized to the centromere of all microchromosomes. W-linked sequences were converted to a single-locus PCR sexing assay. P. vitticeps sex chromosome sequences also shared homology with several other Australian dragons. Further physical mapping and isolation of sex-specific bacterial artificial chromosome clones will provide insight into the evolution of sex determination and sex chromosomes in GSD and TSD dragon lizards.
dc.publisherBlackwell Publishing Ltd
dc.sourceHeredity
dc.subjectKeywords: chromosome; genotype; incubation; lizard; mapping method; phylogeny; polymerase chain reaction; polymorphism; sex determination; temperature effect; amplified fragment length polymorphism; animal; article; chromosome map; chromosome structure; cross breed Agamidae; Sex chromosome; Sex determination; Sex linkage; Sex marker
dc.titleExtension, single-locus conversion and physical mapping of sex chromosome sequences identify the Z microchromosome and pseudo-autosomal region in a dragon lizard, Pogona vitticeps
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume104
dc.date.issued2010
local.identifier.absfor060403 - Developmental Genetics (incl. Sex Determination)
local.identifier.ariespublicationu9511635xPUB545
local.type.statusPublished Version
local.contributor.affiliationQuinn, Alexander E, University of Canberra
local.contributor.affiliationEzaz, Tariq, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationSarre, Stephen, University of Canberra
local.contributor.affiliationGraves, Jennifer, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGeorges, Arthur, University of Canberra
local.description.embargo2037-12-31
local.bibliographicCitation.startpage410
local.bibliographicCitation.lastpage417
local.identifier.doi10.1038/hdy.2009.133
dc.date.updated2016-02-24T12:06:50Z
local.identifier.scopusID2-s2.0-77950077355
local.identifier.thomsonID000275898200012
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01_Quinn_Extension,_single-locus_2010.pdf589.96 kBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator