Directed evolution combined with rational design increases activity of GpdQ toward a non-physiological substrate and alters the oligomeric structure of the enzyme
Directed evolution was used to enhance the activity of the glycerophosphodiesterase enzyme from Enterobacter aerogenes, GpdQ, toward bis(para-nitrophenol) phosphate (BpNPP), a substrate that is frequently used to assay phosphodiesterases. Native GpdQ has
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|Source:||Protein Engineering Design and Selection|
|01_Yip_Directed_evolution_combined_2011.pdf||389.42 kB||Adobe PDF||Request a copy|
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