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Directed evolution combined with rational design increases activity of GpdQ toward a non-physiological substrate and alters the oligomeric structure of the enzyme

Yip, Sylvia; Foo, Jee; Schenk, Gerhard; Gahan, Lawrence; Carr, Paul D; Ollis, David

Description

Directed evolution was used to enhance the activity of the glycerophosphodiesterase enzyme from Enterobacter aerogenes, GpdQ, toward bis(para-nitrophenol) phosphate (BpNPP), a substrate that is frequently used to assay phosphodiesterases. Native GpdQ has

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
URI: http://hdl.handle.net/1885/60509
Source: Protein Engineering Design and Selection
DOI: 10.1093/protein/gzr048

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