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DOTA-Amide Lanthanide Tag for Reliable Generation of Pseudocontact Shifts in Protein NMR Spectra

Graham, Bim; Loh, Choy; Swarbrick, James; Ung, Phuc; Shin, James; Yagi, Hiromasa; Jia, Xinying; Chhabra, Sandeep; Barlow, Nicholas; Pintacuda, Guido; Otting, Gottfried; Huber, Thomas

Description

Structural studies of proteins and protein-ligand complexes by nuclear magnetic resonance (NMR) spectroscopy can be greatly enhanced by site-specific attachment of lanthanide ions to create paramagnetic centers. In particular, pseudocontact shifts (PCS) generated by paramagnetic lanthanides contain important and unique long-range structure information. Here, we present a high-affinity lanthanide binding tag that can be attached to single cysteine residues of proteins. The new tag has many...[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
URI: http://hdl.handle.net/1885/60409
Source: Bioconjugate Chemistry
DOI: 10.1021/bc200353c

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