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Glutamate-354 of the CP43 polypeptide interacts with the oxygen-evolving Mn4Ca cluster of photosystem II: a preliminary characterization of the Glu354Gln mutant

Strickler, Melodie A; Hwang, Hong Jin; Burnap, Robert L; Yano, Junko; Walker, Lee M; Service, Rachel J; Britt, R David; Hillier, Warwick; Debus, Richard John


In the recent X-ray crystallographic structural models of photosystem II, Glu354 of the CP43 polypeptide is assigned as a ligand of the O 2-evolving Mn4Ca cluster. In this communication, a preliminary characterization of the CP43-Glu354Gln mutant of the cyanobacterium Synechocystis sp. PCC 6803 is presented. The steady-state rate of O2 evolution in the mutant cells is only approximately 20% compared with the wild-type, but the kinetics of O2 release are essentially unchanged and the O2-flash...[Show more]

CollectionsANU Research Publications
Date published: 2008
Type: Journal article
Source: Philosophical Transactions of the Royal Society of London Series B
DOI: 10.1098/rstb.2007.2213


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