Evolving improved Synechococcus Rubisco functional expression in Escherichia coli
The photosynthetic CO2-fixing enzyme Rubisco [ribulose-P2 (D-ribulose-1, 5-bisphosphate) carboxylase/ oxygenase] has long been a target for engineering kinetic improvements. Towards this goal we used an RDE (Rubisco-dependent Escherichia coli) selection system to evolve Synechococcus PCC6301 Form I Rubisco under different selection pressures. In the fastest growing colonies, the Rubisco L (large) subunit substitutions 1174V, Q212L, M262T, F345L or F345I were repeatedly selected and shown to...[Show more]
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