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Localization of sucrose synthase in developing seed and siliques of Arabidopsis thaliana reveals diverse roles for SUS during development

Fallahi, Hossein; Schofield, Graham N; Badger, Murray; Chow, Wah S (Fred); Furbank, Robert Thomas; Ruan, Yong-Ling

Description

This study investigated the roles of sucrose synthase (SUS) in developing seeds and siliques of Arabidopsis thaliana. Enzyme activity assays showed that SUS activity was highest in developing whole siliques and young rosette leaves compared with other tissues including mature leaves, stems, and flowers. Surprisingly, quantitative PCR analyses revealed little correlation between SUS activity and transcript expression, which indicated the importance of examining the role of SUS at the protein...[Show more]

dc.contributor.authorFallahi, Hossein
dc.contributor.authorSchofield, Graham N
dc.contributor.authorBadger, Murray
dc.contributor.authorChow, Wah S (Fred)
dc.contributor.authorFurbank, Robert Thomas
dc.contributor.authorRuan, Yong-Ling
dc.date.accessioned2015-12-10T22:55:13Z
dc.identifier.issn0022-0957
dc.identifier.urihttp://hdl.handle.net/1885/60007
dc.description.abstractThis study investigated the roles of sucrose synthase (SUS) in developing seeds and siliques of Arabidopsis thaliana. Enzyme activity assays showed that SUS activity was highest in developing whole siliques and young rosette leaves compared with other tissues including mature leaves, stems, and flowers. Surprisingly, quantitative PCR analyses revealed little correlation between SUS activity and transcript expression, which indicated the importance of examining the role of SUS at the protein level. Therefore, immunolocalization was performed over a developmental time course to determine the previously unreported cellular localization of SUS in Arabidopsis seed and silique tissues. At 3 d and 10 d after flowering (daf), SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 daf, SUS protein was detected in the embryo and aleurone layer, but was absent from the seed coat and funiculus. Starch grains were also present in the seed coat at 3 and 10 daf, but were absent at 13 daf. Co-localization of SUS protein and starch grains in the seed coat at 3 and 10 daf indicates that SUS may be involved in temporary starch deposition during the early stages of seed development, whilst in the later stages SUS metabolizes sucrose in the embryo and cotyledon. Within the silique wall, SUS localized specifically to the companion cells, indicating that SUS activity may be required to provide energy for phloem transport activities in the silique wall. The results highlight the diverse roles that SUS may play during the development of silique and seed in Arabidopsis.
dc.publisherOxford University Press
dc.sourceJournal of Experimental Botany
dc.subjectKeywords: Arabidopsis protein; glucosyltransferase; starch; sucrose synthase; Arabidopsis; article; chemistry; enzymology; flower; gene expression; genetics; growth, development and aging; metabolism; multigene family; plant leaf; plant seed; protein transport; Ara Arabidopsis; Companion cells; Immunolocalization; Nectary; Seed development; Silique wall; Sucrose synthase
dc.titleLocalization of sucrose synthase in developing seed and siliques of Arabidopsis thaliana reveals diverse roles for SUS during development
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume59
dc.date.issued2008
local.identifier.absfor060702 - Plant Cell and Molecular Biology
local.identifier.absfor060199 - Biochemistry and Cell Biology not elsewhere classified
local.identifier.ariespublicationu9204316xPUB517
local.type.statusPublished Version
local.contributor.affiliationFallahi, Hossein, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationSchofield, Graham N, CSIRO
local.contributor.affiliationBadger, Murray, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationChow, Wah S (Fred), College of Medicine, Biology and Environment, ANU
local.contributor.affiliationFurbank, Robert Thomas, CSIRO Division of Plant Industry
local.contributor.affiliationRuan, Yong-Ling, CSIRO Division of Plant Industry
local.description.embargo2037-12-31
local.bibliographicCitation.issue12
local.bibliographicCitation.startpage3283
local.bibliographicCitation.lastpage3295
local.identifier.doi10.1093/jxb/ern180
dc.date.updated2015-12-10T07:48:16Z
local.identifier.scopusID2-s2.0-51749105081
local.identifier.thomsonID000259202900006
CollectionsANU Research Publications

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