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Arabidopsis dynamin-like progein DRP1A: a null mutant with widespread defects in endocytosis, cellulose synthesis, cytokinesis, and cell expansion

Collings, David; Gebbie, L; Howles, Paul; Hurley, Ursula; Birch, Rosemary; Cork, Ann; Hocart, Charles; Arioli, Tony; Williamson, Richard

Description

Dynamin-related proteins are large GTPases that deform and cause fission of membranes. The DRP1 family of Arabidopsis thaliana has five members of which DRP1A, DRP1C, and DRP1E are widely expressed. Likely functions of DRP1A were identified by studying rsw9, a null mutant of the Columbia ecotype that grows continuously but with altered morphology. Mutant roots and hypocotyls are short and swollen, features plausibly originating in their cellulose-deficient walls. The reduction in cellulose is...[Show more]

dc.contributor.authorCollings, David
dc.contributor.authorGebbie, L
dc.contributor.authorHowles, Paul
dc.contributor.authorHurley, Ursula
dc.contributor.authorBirch, Rosemary
dc.contributor.authorCork, Ann
dc.contributor.authorHocart, Charles
dc.contributor.authorArioli, Tony
dc.contributor.authorWilliamson, Richard
dc.date.accessioned2015-12-10T22:53:33Z
dc.identifier.issn0022-0957
dc.identifier.urihttp://hdl.handle.net/1885/59397
dc.description.abstractDynamin-related proteins are large GTPases that deform and cause fission of membranes. The DRP1 family of Arabidopsis thaliana has five members of which DRP1A, DRP1C, and DRP1E are widely expressed. Likely functions of DRP1A were identified by studying rsw9, a null mutant of the Columbia ecotype that grows continuously but with altered morphology. Mutant roots and hypocotyls are short and swollen, features plausibly originating in their cellulose-deficient walls. The reduction in cellulose is specific since non-cellulosic polysaccharides in rsw9 have more arabinose, xylose, and galactose than those in wild type. Cell plates in rsw9 roots lack DRP1A but still retain DRP1E. Abnormally placed and often incomplete cell walls are preceded by abnormally curved cell plates. Notwithstanding these division abnormalities, roots and stems add new cells at wild-type rates and organ elongation slows because rsw9 cells do not grow as long as wild-type cells. Absence of DRP1A reduces endocytotic uptake of FM4-64 into the cytoplasm of root cells and the hypersensitivity of elongation and radial swelling in rsw9 to the trafficking inhibitor monensin suggests that impaired endocytosis may contribute to the development of shorter fatter roots, probably by reducing cellulose synthesis.
dc.publisherOxford University Press
dc.sourceJournal of Experimental Botany
dc.subjectKeywords: Arabidopsis; Arabidopsis thaliana Arabidopsis thaliana; Cell wall; Cellulose synthesis; Cytokinesis; DRP1A; Dynamin-related protein; Endocytosis; FM4-64; Non-cellulosic polysaccharides; Radial swelling mutant
dc.titleArabidopsis dynamin-like progein DRP1A: a null mutant with widespread defects in endocytosis, cellulose synthesis, cytokinesis, and cell expansion
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume59
dc.date.issued2008
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationu9204316xPUB488
local.type.statusPublished Version
local.contributor.affiliationCollings, David, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGebbie, L, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHowles, Paul, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHurley, Ursula, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBirch, Rosemary, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationCork, Ann, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHocart, Charles, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationArioli, Tony, Bayer Bioscience
local.contributor.affiliationWilliamson, Richard, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue2
local.bibliographicCitation.startpage361
local.bibliographicCitation.lastpage376
local.identifier.doi10.1093/jxb/erm324
dc.date.updated2015-12-10T07:35:11Z
local.identifier.scopusID2-s2.0-41649096142
local.identifier.thomsonID000254006500022
CollectionsANU Research Publications

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