Engineering [Ln(DPA) 3 ] 3- binding sites in proteins: a widely applicable method for tagging proteins with lanthanide ions
Paramagnetic relaxation enhancements from unpaired electrons observed in nuclear magnetic resonance (NMR) spectra present powerful long-range distance restraints. The most frequently used paramagnetic tags, however, are tethered to the protein via disulfide bonds, requiring proteins with single cysteine residues for covalent attachment. Here we present a straightforward strategy to tag proteins site-specifically with paramagnetic lanthanides without a tether and independent of cysteine...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of Biomolecular NMR|
|01_Jia_Engineering_[Ln(DPA)_3_]_3-__2011.pdf||450.1 kB||Adobe PDF||Request a copy|
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.