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Engineering [Ln(DPA) 3 ] 3- binding sites in proteins: a widely applicable method for tagging proteins with lanthanide ions

Jia, Xinying; Yagi, Hiromasa; Su, Xun-Cheng; Stanton-Cook, Mitchell; Huber, Thomas; Otting, Gottfried

Description

Paramagnetic relaxation enhancements from unpaired electrons observed in nuclear magnetic resonance (NMR) spectra present powerful long-range distance restraints. The most frequently used paramagnetic tags, however, are tethered to the protein via disulfide bonds, requiring proteins with single cysteine residues for covalent attachment. Here we present a straightforward strategy to tag proteins site-specifically with paramagnetic lanthanides without a tether and independent of cysteine...[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
URI: http://hdl.handle.net/1885/59387
Source: Journal of Biomolecular NMR
DOI: 10.1007/s10858-011-9529-x

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