Skip navigation
Skip navigation

Gadolinium tagging for high-precision measurements of 6 nm distances in protein assemblies by EPR

Yagi, Hiromasa; Banerjee, Debamalya; Graham, Bim; Goldfarb, Daniella; Otting, Gottfried; Huber, Thomas


Double electron-electron resonance (DEER) distance measurements of a protein complex tagged with two Gd3+ chelates developed for rigid positioning of the metal ion are shown to deliver outstandingly accurate distance measurements in the 6 nm range. The accuracy was assessed by comparison with modeled distance distributions based on the three-dimensional molecular structures of the protein and the tag and further comparison with paramagnetic NMR data. The close agreement between the predicted...[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
Source: Journal of the American Chemical Society
DOI: 10.1021/ja204415w


File Description SizeFormat Image
01_Yagi_Gadolinium_tagging_for_2011.pdf1.23 MBAdobe PDF    Request a copy

Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator