Yagi, Hiromasa; Banerjee, Debamalya; Graham, Bim; Huber, Thomas; Goldfarb, Daniella; Otting, Gottfried
Double electron-electron resonance (DEER) distance measurements of a protein complex tagged with two Gd3+ chelates developed for rigid positioning of the metal ion are shown to deliver outstandingly accurate distance measurements in the 6 nm range. The accuracy was assessed by comparison with modeled distance distributions based on the three-dimensional molecular structures of the protein and the tag and further comparison with paramagnetic NMR data. The close agreement between the predicted...[Show more]
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