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Gadolinium tagging for high-precision measurements of 6 nm distances in protein assemblies by EPR

Yagi, Hiromasa; Banerjee, Debamalya; Graham, Bim; Huber, Thomas; Goldfarb, Daniella; Otting, Gottfried

Description

Double electron-electron resonance (DEER) distance measurements of a protein complex tagged with two Gd3+ chelates developed for rigid positioning of the metal ion are shown to deliver outstandingly accurate distance measurements in the 6 nm range. The accuracy was assessed by comparison with modeled distance distributions based on the three-dimensional molecular structures of the protein and the tag and further comparison with paramagnetic NMR data. The close agreement between the predicted...[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
URI: http://hdl.handle.net/1885/59217
Source: Journal of the American Chemical Society
DOI: 10.1021/ja204415w

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