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4,4'-dithiobisdipicolinic acid: a small and convenient lanthanide binding tag for protein NMR spectroscopy

Jia, Xinying; Maleckis, Ansis; Otting, Gottfried; Huber, Thomas


Pseudocontact shifts (PCS) from paramagnetic lanthanide ions present powerful long-range structure restraints for studies of proteins by nuclear magnetic resonance spectroscopy. To elicit PCSs, the lanthanide must be attached site-specifically to the target protein. In addition, it needs to be attached rigidly to avoid averaging of the PCSs due to mobility with respect to the protein and it must not interfere with the function of the protein. Here, we present a dipicolinic acid reagent that...[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
Source: Chemistry, A European Journal
DOI: 10.1002/chem.201003573


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