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A double-emulsion microfluidic platform for in vitro green fluorescent protein expression

Wu, Nan; Oakeshott, John Graham; Easton, Christopher; Peat, Thomas S; Surjadi, Regina; Zhu, Yonggang


Microfluidic droplet technology has gained popularity due to the advantages over conventional emulsion techniques and capabilities for a wide range of applications. In this paper, the development of a simple microfluidic-based double-emulsion system is reported. Such a system could be potentially used for in vitro protein synthesis. The system involves a two-step process to make water-in-oil-in-water (W/O/W) emulsions. A PMMA microchip is used for the formation of water-in-oil (W/O)...[Show more]

CollectionsANU Research Publications
Date published: 2011
Type: Journal article
Source: Journal of Micromechanics and Microengineering
DOI: 10.1088/0960-1317/21/5/054032


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