The assignment of Qy(1,0) vibrational structure and Qx for chlorophyll a
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Hughes, Joseph; Conlan, Brendon; Wydrzynski, Thomas; Krausz, Elmars
Description
We used non-photochemical persistent spectral hole-burning at 1.4 K to investigate the Qy(1,0) vibrational structure of Chl a in a water-soluble chlorophyll-binding protein (WSCP) which exhibits resolved structure in its broadband optical spectra. Franck-Condon vibrational overlap factors were determined from the vibrational hole-burning data and used to simulate the Qy(1,0) spectra. The simulations were not able to accurately reproduce the details of the Qy(1,0) spectrum. This indicates a...[Show more]
dc.contributor.author | Hughes, Joseph | |
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dc.contributor.author | Conlan, Brendon | |
dc.contributor.author | Wydrzynski, Thomas | |
dc.contributor.author | Krausz, Elmars | |
dc.date.accessioned | 2015-12-10T22:50:57Z | |
dc.identifier.issn | 1875-3892 | |
dc.identifier.uri | http://hdl.handle.net/1885/58828 | |
dc.description.abstract | We used non-photochemical persistent spectral hole-burning at 1.4 K to investigate the Qy(1,0) vibrational structure of Chl a in a water-soluble chlorophyll-binding protein (WSCP) which exhibits resolved structure in its broadband optical spectra. Franck-Condon vibrational overlap factors were determined from the vibrational hole-burning data and used to simulate the Qy(1,0) spectra. The simulations were not able to accurately reproduce the details of the Qy(1,0) spectrum. This indicates a breakdown of the approximations used for the analysis and demonstrates that vibrationally induced mixing of electronic states (vibronic coupling) is active for Chl a. By considering the inhomogeneous broadening and vibrational hole-burning phenomena in the Qx and Qy(1,0) region of Chl-WSCP in addition to magnetic circular dichroism data, we favor the traditional placement of Qx at λ∼570-590 nm rather than the alternate assignment underneath the Qy(1,0) absorption near λ∼615-630 nm. | |
dc.publisher | Elsevier | |
dc.source | Physics Procedia | |
dc.subject | Keywords: Binding proteins; Chlorophyll a; Franck-Condon; Franck-Condon factors; Hole burning; Inhomogeneous broadening; Magnetic circular dichroisms; Optical spectra; Persistent spectral hole burning; Vibrational structures; Vibronic coupling; Dichroism; Porphyrin Franck-condon factor; Hole-burning; Inhomogeneous width; Vibronic; WSCP | |
dc.title | The assignment of Qy(1,0) vibrational structure and Qx for chlorophyll a | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 3 | |
dc.date.issued | 2010 | |
local.identifier.absfor | 029901 - Biological Physics | |
local.identifier.ariespublication | u4217927xPUB460 | |
local.type.status | Published Version | |
local.contributor.affiliation | Hughes, Joseph, College of Physical and Mathematical Sciences, ANU | |
local.contributor.affiliation | Conlan, Brendon, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Wydrzynski, Thomas, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Krausz, Elmars, College of Physical and Mathematical Sciences, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 4 | |
local.bibliographicCitation.startpage | 1591 | |
local.bibliographicCitation.lastpage | 1599 | |
local.identifier.doi | 10.1016/j.phpro.2010.01.226 | |
local.identifier.absseo | 970103 - Expanding Knowledge in the Chemical Sciences | |
dc.date.updated | 2016-02-24T10:43:42Z | |
local.identifier.scopusID | 2-s2.0-77951553290 | |
Collections | ANU Research Publications |
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