Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation
The stable incorporation of the intracellular fluorescent dye 5-(and -6)- carboxyfluorescein diacetate succinimidyl ester (CFSE) into cells provides a powerful tool to monitor cell migration, and to quantify cell division, because of the sequential decrease in fluorescent labeling in daughter cells. CFSE-labeled lymphocytes have been used to analyze the relationship between cell division and differentiation of cell function, and cell proliferation versus apoptosis, both in vivo and in vitro,...[Show more]
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|Source:||Current Protocols in Immunology|
|01_Parish_Use_of_the_Intracellular_2009.pdf||195.29 kB||Adobe PDF||Request a copy|
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