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Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation

Parish, Christopher; Clydesdale, Megan; Quah, Ben; Warren, Hilary


The stable incorporation of the intracellular fluorescent dye 5-(and -6)- carboxyfluorescein diacetate succinimidyl ester (CFSE) into cells provides a powerful tool to monitor cell migration, and to quantify cell division, because of the sequential decrease in fluorescent labeling in daughter cells. CFSE-labeled lymphocytes have been used to analyze the relationship between cell division and differentiation of cell function, and cell proliferation versus apoptosis, both in vivo and in vitro,...[Show more]

CollectionsANU Research Publications
Date published: 2009
Type: Journal article
Source: Current Protocols in Immunology
DOI: 10.1002/0471142735.im0409s84


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