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Construction of a tobacco master line to improve Rubisco engineering in chloroplasts

Whitney, Spencer; Sharwood, Robert

Description

The inability to assemble Rubisco from any photosynthetic eukaryote within Escherichia coli has hampered structure-function studies of higher plant Rubisco. Precise genetic manipulation of the tobacco chloroplast genome (plastome) by homologous recombination has facilitated the successful production of transplastomic lines that have either mutated the Rubisco large subunit (L) gene, rbcL, or replaced it with foreign variants. Here the capacity of a new tobacco transplastomic line,cmtrL, to...[Show more]

CollectionsANU Research Publications
Date published: 2008
Type: Journal article
URI: http://hdl.handle.net/1885/58422
Source: Journal of Experimental Botany
DOI: 10.1093/jxb/erm311

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