Construction of a tobacco master line to improve Rubisco engineering in chloroplasts
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Whitney, Spencer; Sharwood, Robert
Description
The inability to assemble Rubisco from any photosynthetic eukaryote within Escherichia coli has hampered structure-function studies of higher plant Rubisco. Precise genetic manipulation of the tobacco chloroplast genome (plastome) by homologous recombination has facilitated the successful production of transplastomic lines that have either mutated the Rubisco large subunit (L) gene, rbcL, or replaced it with foreign variants. Here the capacity of a new tobacco transplastomic line,cmtrL, to...[Show more]
Collections | ANU Research Publications |
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Date published: | 2008 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/58422 |
Source: | Journal of Experimental Botany |
DOI: | 10.1093/jxb/erm311 |
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01_Whitney_Construction_of_a_tobacco_2008.pdf | 707.55 kB | Adobe PDF | Request a copy |
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