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A one-step quantitative reverse transcription polymerase chain reaction procedure

Kelleher, Kim; Leck, Kwong-Joo; Hendry, Ian; Matthaei, Klaus

Description

Our laboratory has developed a one-step quantitative reverse transcription polymerase chain reaction (RT-PCR) procedure in which the reverse transcriptase enzyme and Taq DNA polymerase are combined in the one tube and a single, non-interrupted, thermal cycling program is performed. In the past, RT-PCR has been carried out with two separate steps: (1) reverse transcription of RNA to generate a cDNA pool and (2) polymerase chain reaction amplification of the cDNA. The two-step method can affect...[Show more]

CollectionsANU Research Publications
Date published: 2001
Type: Journal article
URI: http://hdl.handle.net/1885/58175
Source: Brain Research Protocols
DOI: 10.1016/S1385-299X(00)00042-8

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