Kelleher, Kim; Leck, Kwong-Joo; Hendry, Ian; Matthaei, Klaus
Our laboratory has developed a one-step quantitative reverse transcription polymerase chain reaction (RT-PCR) procedure in which the reverse transcriptase enzyme and Taq DNA polymerase are combined in the one tube and a single, non-interrupted, thermal cycling program is performed. In the past, RT-PCR has been carried out with two separate steps: (1) reverse transcription of RNA to generate a cDNA pool and (2) polymerase chain reaction amplification of the cDNA. The two-step method can affect...[Show more]
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.