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31 p MAS-NMR of human erythrocytes: Independence of cell volume from angular velocity

Kuchel, Philip William; Bubb, William A; Ramadan, S; Chapman, B E; Philp, David; Coen, M; Gready, Jill; Harvey, Peta; McLean, Allan J; Hook, J


31P magic angle spinning NMR (MAS-NMR) spectra were obtained from suspensions of human red blood cells (RBCs) that contained the cell-volume-sensitive probe molecule, dimethyl methylphosphonate (DMMP). A mathematical representation of the spectral-peak shape, including the separation and width-at-half-height in the 31P NMR spectra, as a function of rotor speed, enabled us to explore the extent to which a change in cell volume would be reflected in the spectra if it occurred. We concluded that a...[Show more]

CollectionsANU Research Publications
Date published: 2004
Type: Journal article
Source: Magnetic Resonance in Medicine
DOI: 10.1002/mrm.20139


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