Development of a sensitive picrate method to determine total cyanide and acetone cyanohydrin contents of gari from cassava
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The sensitivity of the normal picrate method for determination of total cyanide in cassava was increased tenfold using a small 1 cm2 picrate paper, eluted using 0.5 mL instead of 5 mL of water as in the normal method. The absorbance was measured in a 2 mm cuvette in the spectrophotometer. The sensitive method was calibrated against the normal picrate method. The total cyanide content in mg HCN equivalents/kg sample = ppm, is calculated from the absorbance (A) by the equation ppm = A × 45.7...[Show more]
dc.contributor.author | Bradbury, James | |
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dc.date.accessioned | 2015-12-10T22:41:11Z | |
dc.identifier.issn | 0308-8146 | |
dc.identifier.uri | http://hdl.handle.net/1885/57779 | |
dc.description.abstract | The sensitivity of the normal picrate method for determination of total cyanide in cassava was increased tenfold using a small 1 cm2 picrate paper, eluted using 0.5 mL instead of 5 mL of water as in the normal method. The absorbance was measured in a 2 mm cuvette in the spectrophotometer. The sensitive method was calibrated against the normal picrate method. The total cyanide content in mg HCN equivalents/kg sample = ppm, is calculated from the absorbance (A) by the equation ppm = A × 45.7 which is applicable from 0.1 to 50 ppm. A new method to determine acetone cyanohydrin was developed based on irreversible denaturation of linamarase in 0.1 M HCl at 30 °C for 1 h. Five gari samples from Mozambique gave a mean total cyanide content of 12 ppm (range 6-15 ppm) and mean acetone cyanohydrin content of 11 ppm (range 5-14 ppm). Acetone cyanohydrin liberates cyanide quantitatively in the human intestine. | |
dc.publisher | Elsevier | |
dc.source | Food Chemistry | |
dc.subject | Keywords: acetone; cyanide; cyanohydrin; glucosidase; hydrochloric acid; linamarase; picric acid; unclassified drug; water; article; calibration; cassava; sensitivity analysis; spectrophotometry; Gari; Manihot esculenta Acetone cyanohydrin; Cassava; Gari; Sensitive picrate method; Total cyanide | |
dc.title | Development of a sensitive picrate method to determine total cyanide and acetone cyanohydrin contents of gari from cassava | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 113 | |
dc.date.issued | 2009 | |
local.identifier.absfor | 060101 - Analytical Biochemistry | |
local.identifier.ariespublication | u9511635xPUB414 | |
local.type.status | Published Version | |
local.contributor.affiliation | Bradbury, James, College of Medicine, Biology and Environment, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 4 | |
local.bibliographicCitation.startpage | 1329 | |
local.bibliographicCitation.lastpage | 1333 | |
local.identifier.doi | 10.1016/j.foodchem.2008.08.081 | |
dc.date.updated | 2016-02-24T12:05:02Z | |
local.identifier.scopusID | 2-s2.0-55949120696 | |
Collections | ANU Research Publications |
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