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Management of the diffusion of 4-methylumbelliferone across phases in microdroplet-based systems for in vitro protein evolution

Wu, Nan; Courtois, Fabienne; Zhu, Yonggang; Oakeshott, John Graham; Easton, Christopher; Abell, Christopher


Fluorongenic reagents based on 4-methylumbelliferone (4-MU) have been widely used for the detection of phosphatase, sulfatase, esterase, lipase and glycosidase activities in conventionally formatted enzyme assay systems. However, the sensitivity of assays based on these substrates is also potentially very useful in the microdroplet formats now being developed for high throughput in vitro evolution experiments. In this article, we report the investigation of diffusion of 4-MU as a model dye from...[Show more]

CollectionsANU Research Publications
Date published: 2010
Type: Journal article
Source: Electrophoresis
DOI: 10.1002/elps.201000140


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