Quantitative PCR measurements of the effects of introducing inosines into primers provides guidelines for improved degenerate primer design
Polymerase chain reaction (PCR) is used to detect groups of viruses with the use of group-specific degenerate primers. Inosine residues are sometimes used in the primers to match variable positions within the complementary target sequences, but there is little data on their effects on cDNA synthesis and amplification. A quantitative reverse-transcription PCR was used to measure the rate of amplification with primers containing inosine residues substituted at different positions and in...[Show more]
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|Source:||Journal of Virological Methods|
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