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Differences in Carbon Isotope Discrimination of Three Variants of D-Ribulose-1,5-biophosphate Carboxylase/Oxygenase Reflect Differences in Their Catalytic Mechanisms

McNevin, Dennis; Badger, Murray; Whitney, Spencer; von Caemmerer, Susanne; Tcherkez, Guillaume; Farquhar, Graham


The carboxylation kinetic (stable carbon) isotope effect was measured for purified D-ribulose-1,5-bisphosphate carboxylases/oxygenases (Rubiscos) with aqueous CO2 as substrate by monitoring Rayleigh fractionation using membrane inlet mass spectrometry. This resulted in discriminations (Δ) of 27.4 ± 0.9‰ for wild-type tobacco Rubisco, 22.2 ± 2.1‰ for Rhodospirillum rubrum Rubisco, and 11.2 ± 1.6‰ for a large subunit mutant of tobacco Rubisco in which Leu335 is mutated to valine (L335V). These Δ...[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
Source: Journal of Biological Chemistry
DOI: 10.1074/jbc.M706274200


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