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Human Glutathione Transferase T2-2 Discloses Some Evolutionary Strategies for Optimization of the Catalytic Activity of Glutathione Transferases

Caccuri, Anna Maria; Antonini, Giovanni; Board, Philip; Flanagan, Jack U; Parker, Michael William; Paolesse, Roberto; Turella, Paola; Chelvanayagam, Gareth; Ricci, G


Steady state, pre-steady state kinetic experiments, and site-directed mutagenesis have been used to dissect the catalytic mechanism of human glutathione transferase T2-2 with 1-menaphthyl sulfate as co-substrate. This enzyme is close to the ancestral precursor of the more recently evolved glutathione transferases belonging to Alpha, Pi, and Mu classes. The enzyme displays a random kinetic mechanism with very low kcat and k cat/ Km(GSH) values and with a rate-limiting step identified as the...[Show more]

CollectionsANU Research Publications
Date published: 2001
Type: Journal article
Source: Journal of Biological Chemistry
DOI: 10.1074/jbc.M002818200


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