Skip navigation
Skip navigation

Directed Evolution of Rubisco in Escherichia coli Reveals a Specificity-Determining Hydrogen Bond in the Form 11 Enzyme

Mueller-Cajar, Oliver; Morell, Matthew; Whitney, Spencer


Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) occupies a critical position in photosynthetic CO2-fixation and consequently has been the focus of intense study. Crystal-structure-guided site-directed mutagenesis studies have met with limited success in engineering kinetic improvements to Rubisco, highlighting our inadequate understanding of structural constraints at the atomic level that dictate the enzyme's catalytic chemistry. Bioselection provides an alternative random mutagenic...[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
Source: Biochemistry
DOI: 10.1021/bi700820a


File Description SizeFormat Image
01_Mueller-Cajar_Directed_Evolution_of_Rubisco_2007.pdf1.38 MBAdobe PDF    Request a copy

Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  12 November 2018/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator