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Directed Evolution of Rubisco in Escherichia coli Reveals a Specificity-Determining Hydrogen Bond in the Form 11 Enzyme

Mueller-Cajar, Oliver; Morell, Matthew; Whitney, Spencer

Description

Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) occupies a critical position in photosynthetic CO2-fixation and consequently has been the focus of intense study. Crystal-structure-guided site-directed mutagenesis studies have met with limited success in engineering kinetic improvements to Rubisco, highlighting our inadequate understanding of structural constraints at the atomic level that dictate the enzyme's catalytic chemistry. Bioselection provides an alternative random mutagenic...[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
URI: http://hdl.handle.net/1885/57196
Source: Biochemistry
DOI: 10.1021/bi700820a

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