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Dichloromethane mediated in vivo selection and functional characterization of rat glutathione S-transferase theta 1-1 variants

Gisi, Daniel; Maillard, Julien; Flanagan, Jack U; Rossjohn, J; Chelvanayagam, Gareth; Board, Philip; Parker, Michael William; Leisinger, Thomas; Vuilleumier, Stephane


Methylobacterium dichloromethanicum DM4 is able to grow with dichloromethane as the sole carbon and energy source by using a dichloromethane dehalogenase/glutathione S-transferase (GST) for the conversion of dichloromethane to formaldehyde. Mammalian homologs of this bacterial enzyme are also known to catalyze this reaction. However, the dehalogenation of dichloromethane by GST T1-1 from rat was highly mutagenic and toxic to methylotrophic bacteria. Plasmid-driven expression of rat GST T1-1 in...[Show more]

CollectionsANU Research Publications
Date published: 2001
Type: Journal article
Source: European Journal of Biochemistry
DOI: 10.1046/j.1432-1327.2001.02314.x


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