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Quantification of tissue inhibitor of metalloproteinases 2 in plasma from healthy donors and cancer patients

Larsen, M.B.; Stephens, Ross; Brunner, N; Nielsen, H.J.; Engelholm, L.H.; Christensen, I.J.; Stetler-Stevenson, W.G.; Høyer-Hansen, G


Tissue inhibitor of metalloproteinases (TIMP)-2 is a highly conserved molecule, which binds both active and latent matrix metalloproteinase (MMP)-2. TIMP-2 is also involved in the activation of MMP-2 on the cell surface. A quantitative enzyme-linked immunosorbent assay (ELISA) was established and optimized for measurement of TIMP-2 in plasma. The capturing antibody in the ELISA was a monoclonal, while the detecting antibody was a chicken polyclonal antibody recognizing the native form of human...[Show more]

CollectionsANU Research Publications
Date published: 2005
Type: Journal article
Source: Scandinavian Journal of Immunology
DOI: 10.1111/j.1365-3083.2005.01585.x


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