Glutarate and N -acetyl-L-glutamate buffers for cell-free synthesis of selectively 15 N-labelled proteins
Cell-free protein synthesis provides rapid and economical access to selectively 15N-labelled proteins, greatly facilitating the assignment of 15N-HSQC spectra. While the best yields are usually obtained with buffers containing high concentrations of potassium L-glutamate, preparation of selectively 15N-Glu labelled samples requires non-standard conditions. Among many compounds tested to replace the L-Glu buffer, potassium N-acetyl-L-glutamate and potassium glutarate were found to perform best,...[Show more]
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|Source:||Journal of Biomolecular NMR|
|01_Jia_Glutarate_and__N_2009.pdf||388.22 kB||Adobe PDF||Request a copy|
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