Skip navigation
Skip navigation

Glutarate and N -acetyl-L-glutamate buffers for cell-free synthesis of selectively 15 N-labelled proteins

Jia, Xinying; Ozawa, Kiyoshi; Loscha, Karin; Otting, Gottfried


Cell-free protein synthesis provides rapid and economical access to selectively 15N-labelled proteins, greatly facilitating the assignment of 15N-HSQC spectra. While the best yields are usually obtained with buffers containing high concentrations of potassium L-glutamate, preparation of selectively 15N-Glu labelled samples requires non-standard conditions. Among many compounds tested to replace the L-Glu buffer, potassium N-acetyl-L-glutamate and potassium glutarate were found to perform best,...[Show more]

CollectionsANU Research Publications
Date published: 2009
Type: Journal article
Source: Journal of Biomolecular NMR
DOI: 10.1007/s10858-009-9315-1


File Description SizeFormat Image
01_Jia_Glutarate_and__N_2009.pdf388.22 kBAdobe PDF    Request a copy

Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator