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Chaperonin-encapsulation of proteins for NMR

Tanaka, Shinji; Kawata, Yasushi; Dixon, Nicholas; Matsuzaki, Katsumi; Hoshino, Masaru; Otting, Gottfried


A novel chaperonin-encapsulation system for NMR measurements has been designed. The single-ring variant SR398 with an ATPase deficient mutation of GroEL, also known as chaperonin, bound co-chaperonin GroES irreversibly, forming a stable cage to encapsulate a target protein. A small GroEL-binding tag made it possible to perform all steps of the encapsulation under near physiological conditions while retaining the native conformation of the target protein. About half of the SR398/GroES cages...[Show more]

CollectionsANU Research Publications
Date published: 2010
Type: Journal article
Source: Biochimica et Biophysica Acta: Proteins & Proteomics
DOI: 10.1016/j.bbapap.2009.12.016


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