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Effects of root restriction on growth and associated cytokinin levels in cotton (Gossypium hirsutum)

Yong, Jean; Letham, D Stuart; Wong, Suan Chin; Farquhar, Graham

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Root restriction experiments on cotton plants were conducted under conditions where water and nitrogen supplies were not limiting. Xylem sap and leaf tissues were obtained before significant biomass reduction as a result of root restriction. Xylem-derived cytokinins (CTKs) entering a leaf and the CTK levels in the same excised leaf were measured, thus, enabling xylem sap and foliar CTKs to be compared concurrently under root-restrictive and control conditions. Abscisic acid (ABA) was also...[Show more]

dc.contributor.authorYong, Jean
dc.contributor.authorLetham, D Stuart
dc.contributor.authorWong, Suan Chin
dc.contributor.authorFarquhar, Graham
dc.date.accessioned2015-12-10T22:34:46Z
dc.identifier.issn1445-4408
dc.identifier.urihttp://hdl.handle.net/1885/56011
dc.description.abstractRoot restriction experiments on cotton plants were conducted under conditions where water and nitrogen supplies were not limiting. Xylem sap and leaf tissues were obtained before significant biomass reduction as a result of root restriction. Xylem-derived cytokinins (CTKs) entering a leaf and the CTK levels in the same excised leaf were measured, thus, enabling xylem sap and foliar CTKs to be compared concurrently under root-restrictive and control conditions. Abscisic acid (ABA) was also measured simultaneously in the same xylem sap samples. Root restriction reduced photosynthesis and18O of leaf matter showed that leaves of root-restricted plants generally had lower stomatal conductances. Root-restriction significantly decreased (37%) the delivery rate per unit leaf area of CTK bases (dihydrozeatin (DZ) and N6-(2-isopentenyl)adenine (iP)) to the leaf but did not alter the export of CTK O-glucosides and ABA from the roots to leaf 4. Root-restriction also did not alter significantly the levels of total CTK bases in the leaves but increased (54-57% higher) the CTK O-glucosides (especially zeatin O-glucosides). Root restriction also slowed down the apparent CTK turnover rates in the leaves. The slow down in CTK turnover rate can be interpreted as a reduction in leaf CTK metabolism during root restriction.
dc.publisherCSIRO Publishing
dc.sourceFunctional Plant Biology
dc.subjectKeywords: Abscisic acid; root pressure chamber; root-shoot signalling; Stable isotopes; trans-zeatin; Xylem sap; Acids; Carbohydrates; Ecology; Endocrinology; Hormones; Isotopes; Plants (botany); Cotton; abscisic acid; cotton; growth rate; growth response; hormone; abscisic acid; hormones; root pressure chamber; root-shoot signalling; stable isotopes; trans-zeatin; xylem sap
dc.titleEffects of root restriction on growth and associated cytokinin levels in cotton (Gossypium hirsutum)
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume37
dc.date.issued2010
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationf2965xPUB348
local.type.statusPublished Version
local.contributor.affiliationYong, Jean, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationLetham, D Stuart, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationWong, Suan Chin, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationFarquhar, Graham, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue10
local.bibliographicCitation.startpage974
local.bibliographicCitation.lastpage984
local.identifier.doi10.1071/FP10009
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2016-02-24T08:27:38Z
local.identifier.scopusID2-s2.0-77957781403
local.identifier.thomsonID000282089000011
CollectionsANU Research Publications

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