O'Keefe, Louise V; Smibert, Peter; Colella, Alex; Chataway, Tim K; Saint, Robert; Richards, Robert I
The generation and analysis of mutants is central to studies of gene function in model organisms. Methods for random mutagenesis in Drosophila melanogaster have been available for many years, but an alternative approach - targeted mutagenesis using homologous recombination - has only recently been developed. This approach has the advantage of specificity, because genes of interest can be altered. One might expect with a gene-targeting approach that the frequency of background mutations would be...[Show more]
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