Structure-based rational design of a phosphotriesterase
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Jackson, Colin J; Weir, Khali; Herlt, Anthony; Khurana, Jeevan; Sutherland, Tara D; Horne, Irene; Easton, Christopher; Russell, Robyn; Scott, Colin; Oakeshott, John Graham
Description
In silico substrate docking of both stereoisomers of the pesticide chlorfenvinphos (CVP) in the phosphotriesterase from Agrobacterium radiobacter identified two residues (F131 and W132) that prevent productive substrate binding and cause stereospecificity. A variant (W131H/F132A) was designed that exhibited ca. 480-fold and 8-fold increases in the rate of Z-CVP and E-CVP hydrolysis, respectively, eliminating stereospecificity.
Collections | ANU Research Publications |
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Date published: | 2009 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/55488 |
Source: | Applied and Environmental Microbiology |
DOI: | 10.1128/AEM.00629-09 |
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