Skip navigation
Skip navigation

[Ln(DPA) 3 ] 3- is a convenient paramagnetic shift reagent for protein NMR studies

Su, Xun-Cheng; Liang, Haobo; Loscha, Karin; Otting, Gottfried

Description

(Chemical Equation Presented) Paramagnetic lanthanide ions present outstanding tools for structural biology by NMR spectroscopy. Here we show that the 3:1 complexes between dipicolinic acid and lanthanides are paramagnetic reagents which can site-specifically bind to a wide range of proteins without formation of a covalent bond. The observed pseudocontact shifts can be interpreted by a single magnetic susceptibility anisotropy tensor, enabling its use for structure refinements. The resonance...[Show more]

CollectionsANU Research Publications
Date published: 2009
Type: Journal article
URI: http://hdl.handle.net/1885/55387
Source: Journal of the American Chemical Society
DOI: 10.1021/ja9034957

Download

File Description SizeFormat Image
01_Su_[Ln(DPA)_3_]_3-__is_a_2009.pdf869.08 kBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  12 November 2018/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator