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NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B-NS3 protease

Su, Xun-Cheng; Ozawa, Kiyoshi; Yagi, Hiromasa; Lim, Siew P; Wen, Daying; Ekonomiuk, Dariusz; Huang, Danzhi; Keller, Thomas H; Sonntag, Sebastian; Caflisch, Amedeo; Vasudevan, Subhash G.; Otting, Gottfried


The two-component NS2B-NS3 protease of West Nile virus is essential for its replication and presents an attractive target for drug development. Here, we describe protocols for the high-yield expression of stable isotope-labelled samples in vivo and in vitro. We also describe the use of NMR spectroscopy to determine the binding mode of new low molecular mass inhibitors of the West Nile virus NS2B-NS3 protease which were discovered using high-throughput in vitro screening. Binding to the...[Show more]

CollectionsANU Research Publications
Date published: 2009
Type: Journal article
Source: The FEBS Journal
DOI: 10.1111/j.1742-4658.2009.07132.x


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