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A fluorometric method to quantify protein glutathionylation using glutathione derivatization with 2,3- naphthalenedicarboxaldehyde

Menon, Deepthi; Board, Philip

Description

This study reports the development of a new assay for the rapid determination of protein glutathionylation in tissues and cell lines using commercially available reagents and standard instrumentation. In this method cells are homogenized in the presence of N-ethylmaleimide to eliminate free thiols and the proteins are precipitated with acetone. Subsequently, the disulfide-bound glutathione is eluted from the protein by the addition of tris(2-carboxyethyl)phosphine and reacted with...[Show more]

CollectionsANU Research Publications
Date published: 2013
Type: Journal article
URI: http://hdl.handle.net/1885/55180
Source: Analytical Biochemistry
DOI: 10.1016/j.ab.2012.10.009

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