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Effect of the Air-Water Interface on the Structure of Lysozyme in the Presence of Guanidinum Chloride

Perriman, Adam; Henderson, Mark; Evenhuis, Christian; McGillivray, Duncan; White, John


We report observations of the changes in the surface structure of lysozyme adsorbed at the air-water interface produced by the chemical denaturant guanidinium chloride. A primary result is the durability of the adsorbed surface layer to denaturation, as compared to the molecule in the bulk solution. Data on the surface film were obtained from X-ray and neutron reflectivity measurements and modeled simultaneously. The behavior of lysozyme in G.HC1 solutions was determined by small-angle X-ray...[Show more]

CollectionsANU Research Publications
Date published: 2008
Type: Journal article
Source: Journal of Physical Chemistry B
DOI: 10.1021/jp800354r


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